Fig. 4

Silencing PinX1 potentiates the antitumor efficacy of radioimmunotherapy. A Experimental schedule of varied treatment regimens for LLC tumor-bearing mice. B Representative photos of tumor xenografts 14 days after the initiation of IR. C Tumor volume of xenografts was shown in the graphs. Two-way ANOVA with Tukey’s multiple comparison was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group. D, E Representative flow cytometry staining and frequencies of total CD8⁺/CD3⁺ cells in tumors. n = 5 mice/group. F, G Representative flow cytometry staining and frequencies of PD-1⁺ Tim-3⁺/CD8⁺ T cells in tumors. n = 5 mice/group. H–J. Representative flow cytometry staining and frequencies of CD86⁺CD206⁻/CD45⁺F4/80⁺CD11b⁺ cells and CD86⁻CD206⁺/CD45⁺F4/80⁺CD11b⁺ cells cells in tumors. n = 5 mice/group. Data are presented as the mean ± standard deviation. *: p < 0.05, **: p < 0.01, ***:p < 0.001, ****:p < 0.0001