Fig. 3

Silencing PinX1 combined with IR increased CD8⁺ T cell recruitment. A Lewis cells were transfected with a control or shPinX1 lentivirus and analyzed by western blotting. B. Experimental schedule of varied treatment regimens for LLC tumor-bearing mice. C Representative photos of tumor xenografts 14 days after the initiation of IR. D Tumor volume of xenografts was shown in the graphs. Two-way ANOVA with Tukey’s multiple comparison test was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group. E Survival data were compared using log-rank Mantel-Cox test. n = 8 mice/group. F–K Representative flow cytometry staining and frequencies of total CD8⁺/CD3⁺ cells, PD-1⁺ Tim-3⁺/CD8⁺ T cells, and Gzmb⁺ /CD8⁺ T cells in tumors. n = 5 mice/group. Data are presented as the mean ± standard deviation, *: p < 0.05, **: p < 0.01, ***p: < 0.001, ****: p < 0.0001