Fig. 1

Silencing PinX1 enhances NSCLC radiosensitivity in vitro and in vivo. A A549 and H460 cells were transfected with a control or shPinX1 lentivirus and analyzed by western blotting. B Cell viability was analyzed via Cell Counting Kit-8 (CCK-8) assays. C Representative images of colony formation of cells irradiated with 0, 2, 4, and 6 Gy. D The cell survival curves were fitted with the linear-quadratic model. E When the tumor grew to a volume of approximately 200 mm3, mice were randomly divided into the following four groups: NC (negative control), shPinX1, IR, and IR plus shPinX1 (n = 5 mice/group). Experimental schedule of shPinX1 and IR combination treatment for A549 tumor-bearing nude mice. F. Representative photos of tumor xenografts 14 days after the initiation of IR. G Xenograft tumor volumes are shown in the graphs. Two-way ANOVA with Tukey’s multiple comparison test was conducted for tumor volume analysis with GraphPad Prism 8.0.2. Data are presented as the mean ± standard deviation. H Survival data were compared using the log-rank Mantel-Cox test.(n = 8 mice/group). *: p < 0.05, **: p < 0.01, ***p: < 0.001, ****: p < 0.0001