Fig. 1

Morn2 knockout causes male infertility. A Overview of the Morn2^–/– mouse knockout strategy. B Genotyping analysis using lysates prepared from the tails of Morn2^–/–, Morn2^+/+, and Morn2^+/– mice showed successful knockout of Morn2. C Representative photographs of testes of Morn2^+/– and Morn2^–/– mice. D The body weight of adult Morn2^+/– and Morn2^–/– mice. Data are shown as the mean ± SEM of 15 independent experiments, each data point represents the body weight of an individual, and ns = P > 0.05 by Student’s t-test. E The testes weight of adult Morn2^+/– and Morn2^–/– mice. Data are shown as the mean ± SEM of 15 independent experiments, each data point represents the testes weight of an individual, and ns = P > 0.05 by Student’s t-test. F Quantification of the testes weight/body weight ratio in Morn2^+/– and Morn2^–/– mice. Data are shown as the mean ± SEM of 15 independent experiments, each data point represents the testis-to-body weight ratio of an individual, and ns = P > 0.05 by Student’s t-test. G Number of pups per litter from Morn2^+/– and Morn2^–/– male mice. In Morn2^+/–mice, the mean number of pups per litter from 10 litters was 7.40 ± 1.90. In Morn2^–/– mice, no pups were born. Data are shown as the mean ± SEM of 3 independent experiments, each data point represents the number of pups per litter of an individual, and **** = P < 0.0001 by Student’s t-test