Fig. 3
Cellular response in 3D cell culture. a NGR_fit curves obtained by treating the 4 cell lines in 3D with lorlatinib. b Repotrectinib. c Entrectinib d Ceritinib and e Crizotinib. f Summary of the AOC values for all treatments and cell lines revealing a resistant phenotype of ROS1G2032R spheroids towards lorlatinib (p < 0.001), repotrectinib (p < 0.001), entrectinib (p < 0.001), ceritinib (p < 0.001) and crizotinib (p < 0.001). ROS1L2026M spheroids showed resistance against lorlatinib (p < 0.001), repotrectinib (p < 0.001) and entrectinib (p < 0.001) but displayed an increased sensitivity towards crizotinib (p = 0.04). Results are the summary of 3 technical replicates combined