Fig. 1
Cellular response in 2D cell culture: a Normalized growth rate (NGR_fit) curves obtained by treating the 4 cell lines in 2D with lorlatinib. b Repotrectinib. c Entrectinib d Ceritinib and e Crizotinib. f Summary of the area over the curve (AOC) values for all treatments and cell lines. ROS1G2032R mutant cells were significantly resistant to lorlatinib (p = 0.01), entrectinib (p < 0.001), ceritinib (p < 0.001) and crizotinib (p < 0.001). ROS1L2026M showed a significant resistance towards crizotinib (p) as well as ROS1S1986Y cells when treated in 2D culture (p). g NGR_fit curves and bar plot summarizing AOC values in 2D-treated ROS1 wild type cells. No significant differences in AOC across different TKI treatments were detected. Results are the summary of 4 biological replicates combined