Fig. 3

Immunohistochemistry with 2C5 sdAb and reference antibodies on human brain slices from AD and control patients. Immunohistochemistry was performed with the following reference antibodies: Tau AT8 (A), Tau T22 (B, C), and amyloid Aβ 4G8 (D) in the temporal cortex of a AD Braak V subject (A, B, D) and control Braak I (C). (E) Immunohistochemistry in the AD (Braak V) subject with the 2C5 sdAb, revealing staining that resembles Tau AT8, Tau T22 and 4G8 antibodies. (F) Immunohistochemistry in the temporal cortex of a control subject with the 2C5 sdAb, revealing low intracellular staining. Black arrows identify neurites and neuron cell bodies, black stars localise extracellular deposits. Slices were hematoxylin counterstained. Scale bars were indicated in each image