Fig. 8

RGS6 knockdown in heart prevents doxorubicin-dependent changes in Nucleolin and miRNA-21 expression and activity. Intracardiac injections of scramble or RGS6 specific shRNA (RGS6 KD) were administered to mice aged 1-week and followed by treatment with doxorubicin (cumulative dose of 45 mg/kg 9 mg/kg, i.p. every other week) or saline for 10 weeks beginning at age 8–10 weeks. Samples were collected 1 week after the last dose later for biochemical and histological analyses. A Representative immunoblots with quantification for RGS6, Nucleolin, pNucleolin (pT76), Nucleophosmin, β-MHC, ANP, Troponin T, and MDA (n = 6). B Fold change inpre-rRNA, (n = 3). C miRNA-21expression (n = 3). Expression of miRNA-21 target genes D TP63 and E PPARα (n = 3). F Representative immunoblots for miRNA-21 targets TP63, Bcl-2, PPARα, and PTEN (n = 6). β-Actin served as the loading control for the immunoblots. Data were analyzed by two-way ANOVA with Sidak’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM