Fig. 7
From: RGS6 drives cardiomyocyte death following nucleolar stress by suppressing Nucleolin/miRNA-21
Inhibition of miRNA-21 or Nucleolin depletion phenocopies the impact of RGS6 on nucleolar stress. A–D Murine VCMs were transduced with scramble or Nucleolin specific shRNA (Nucleolin KD) ± doxorubicin (3 μM, 16 h). A Representative immunoblots and quantification for RGS6, Nucleolin, pNucleolin (pT76), and Nucleophosmin (n = 3). B Fold change in pre-rRNA (n = 3). C Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). D MTT assay to determine cell viability (n = 6). E–H Murine VCMs were transduced with scramble or miRNA-21 inhibitor (miRNA-21i) ± doxorubicin (3 μM, 16 h). E Immunoblots and quantification for RGS6, Nucleolin, pNucleolin (pT76), and Nucleophosmin (n = 3). F Fold change in pre-rRNA (n = 3). G Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). H MTT assay to determine cell viability (n = 6). β-Actin served as the loading control for the immunoblots. Data were analyzed by two-way ANOVA with Sidak’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM