Fig. 6

Doxorubicin-dependent nucleolar stress requires RGS6. A immunoblots for RGS6, Nucleolin, and Nucleophosmin with quantification (n = 3), in Control or RGS6 KD AC-16 cells ± doxorubicin (3 µM, 16 h). B–E AC-16 cells were transduced with RGS6 specific shRNA (RGS6 KD) or scramble shRNA ± doxorubicin (3 μM, 16 h). B Fold change in pre-rRNA (n = 3). C Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). D MTT assay to determine cell viability (n = 6). E Immunoblots with quantification for RGS6, Nucleolin, pNucleolin (pT76), Nucleophosmin, β-MHC, MDA, 4HNE, and Bcl-2 (n = 6). β-Actin served as a loading control for immunoblots. Data were analyzed by two-way ANOVA with Sidak’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM