Fig. 5

Chemotherapy exposure is associated with parallel changes in RGS6 and Nucleolin/Nucleophosmin expression in heart. A Representative staining and quantification of cardiac RGS6 and Nucleolin expression in control (n = 10) or patients with a history of chemotherapy (n = 10) [scale bar = 100 μm]. B Representative immunoblots and quantification for RGS6, Nucleolin and Nucleophosmin in the heart of control patients (n = 10) or those with a history of chemotherapy exposure (n = 14). C, D Mice were treated with saline control (n = 10) or doxorubicin(cumulative dose of 45 mg/kg 9 mg/kg, i.p. every other week) for 10 weeks (C) (n = 10), (D) (n = 14). Samples were collected 1 week after the last dose for biochemical and histological analyses. (C)Representative staining and quantification of cardiac RGS6 and Nucleolin expression [scale bar = 100 μm]. D Representative immunoblots and quantification for RGS6, Nucleolin, and Nucleophosmin (n = 14). β-Actin served as a loading control for immunoblots. Data were analyzed by two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM