Fig. 4

RGS6 controls expression of Nucleolin and miRNA-21 in vivo. An RGS6 encoding viral construct (RGS6 OE) or vector control was introduced into the myocardium of mice aged 1-week. After allowing the virus to be expressed for 10–12 days, animals were sacrificed, and tissues collected for downstream analyses. A Representative immunoblots with quantification for RGS6, Nucleolin, pNucleolin (pT76), and Nucleophosmin, (n = 12). B miRNA-21 expression (n = 3). C Fold change inpre-rRNA(n = 3). D mRNA expression of miRNA-21targetsTP63, FASL, PTEN, BCL2, and PPARα (n = 3). E Representative immunoblots with quantification for miRNA-21 targets TP63, FASL, PTEN, Bcl-2, and PPARα, (n = 12). β-Actin served as a loading control for immunoblots. Data were analyzed by two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM