Fig. 3
From: RGS6 drives cardiomyocyte death following nucleolar stress by suppressing Nucleolin/miRNA-21
RGS6 controls expression of miRNA-21 via a Nucleolin-dependent mechanism. A, B AC-16 cells were transduced with Nucleolin-HA (Nucleolin OE), RGS6-pEGFP (RGS6 OE), both constructs, or vector control and harvested 36 hours after transduction. A miRNA-21 expression levels (n = 3). B mRNA levels of miRNA-21 target genes TP63, FASL, PPARα, BCL2, PTEN (n = 3). C–F AC-16 cells were transduced with miRNA-21 (miRNA-21 OE), RGS6-pEGFP (RGS6 OE), both constructs, or vector control and harvested after 36 h). C Representative immunoblots with quantification for γH2AX, Bax, PUMA, 4HNE and MDA. β-Actin served as a loading control for immunoblots. D Validation of miRNA-21 overexpression (n = 3). E Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). F CM-H2-DCFDA fluorescence (ROS; n = 5). Data were analyzed by two-way ANOVA with Sidak’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented 0.001, ± SEM