Fig. 2
From: RGS6 drives cardiomyocyte death following nucleolar stress by suppressing Nucleolin/miRNA-21
RGS6 promotes nucleolar stress-driven cell death by down-regulating Nucleolin. A–C Murine VCMs were transduced with scramble or RGS6 specific shRNA ± Actinomycin D (5 µM 16 h). A Representative immunoblots with quantification for Nucleolin, pNucleolin (pT76), and Nucleophosmin (n = 6). B Fold change in pre-rRNA levels (n = 3). C Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). (D-H) Murine VCMs were transduced with Nucleolin-HA (Nucleolin OE), RGS6-pEGFP (RGS6 OE), both constructs, or vector control and harvested 36 hours after transduction. D Mitochondrial membrane potential (ΔΨM; n = 5). E Mitochondrial Ca2+ Flux (n = 5). F CM-H2-DCFDA fluorescence (ROS; n = 5). G Apoptosis (cytoplasmic histone-associated DNA fragments; n = 5). H Fold change in pre-rRNA levels (n = 3). I Representative immunoblots and quantification for γH2AX, Bax, PUMA, 4-HNE and MDA.β-Actin served as a loading control for immunoblots. Data were analyzed by two-way ANOVA with Sidak’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM