Fig. 7

DSPE-PEG-CMP-miR302-EXO treatment significantly reduced myocardial apoptosis. A TUNEL staining (Nuclei was stained blue with DAPI) demonstrated that cardiomyocytes underwent apoptosis. Scale bar = 100 μm. B DSPE-PEG-CMP-miR302-EXO reduced the apoptotic rate of cardiomyocyte (TUNEL-positive cells (%) are expressed as number of apoptotic myocytes/total myocytes). C–E Western blot analysis showed that DSPE-PEG-CMP-miR302-EXO upregulated Bcl-2 expression but inhibited that of Bax in cardiomyocytes. GAPDH served as the internal reference. Values are expressed as mean ± SD (n = 3). a: Control; b: Model; c: DSPE-PEG-CMP; d: DSPE-PEG-CMP-EXO; e: DSPE-PEG-CMP-miR302-EXO; f: miR302. ^▲▲p < 0.01 vs control; *p < 0.05, **p < 0.01 vs Model; ^##p < 0.01 vs DSPE-PEG-CMP-EXO; ^&&p < 0.01 vs miR302