Fig. 5

The function of Trm_gut_CD8 from distinct intestine organs in colorectal cancer and its clinical significance. A tSNE plots indicating the mapping and annotation from reference dataset to mapped scRNA-seq dataset including T cells of paired tumor and normal samples. B The dot plot indicating the expression levels of marker genes among predicted T cell subsets derived from distinct organs. C The bar plot indicates the proportion of predicted T cell subset between paired tumor and normal samples. D The differential genes of tumor Trm_gut_CD8 subsets of distinct organs compared to the normal sample. Genes with log2Foldchange greater than 0.5 and p value adjust less than 0.001 were annotated as differential genes. E The bar plot indicating the enriched signature of genes mentioned in (d). F Overall survival curves of colorectal cancer patients stratified by the infiltration scores of Tmr_gut_CD8 derived from distinct intestine organs. G Box plots indicating the correlation between the infiltration of Trm_gut_CD8 cells of distinct organs and the response to the immune checkpoint blockade therapy in the colorectal scRNA-seq data