Fig. 4

Inhibition of the TINAGL1/integrin/FAK axis decreased the CAF-induced aggressiveness of DGC. A TINAGL1 gene and protein levels in siRNA-transfected CAF47 by RT-PCR and western blotting. B Western blotting for FAK phosphorylation in siRNA-transfected CAF47-co-cultured SNU601 cells. C, D Graph (C) and representative images (D) of the transwell migration assay of siRNA-transfected CAF47-co-cultured SNU601 cells (magnification, × 100). Data were analyzed using the Kruskal–Wallis test with Dunn’s test. E Western blotting for FAK phosphorylation in PF-573,228-treated SNU601 cells. F, G Graph (F) and representative images (G) of the transwell migration assay of PF-573,228-treated SNU601 cells (magnification, × 100). Data were analyzed using the Kruskal–Wallis test with Dunn’s test. H RT-PCR for TINAGL1 expression in shRNA-transfected immortalized CAF47. I Representative image for subcutaneously injected SNU601 xenograft tumors. The small tissue clusters under each large tumor tissue are metastasized inguinal lymph nodes. J Representative image for cytokeratin staining, and the pie chart shows the number of tumors with lymph node metastasis. *P < 0.05, **P < 0.01