Fig. 3

CAF-secreted TINAGL1 increased the aggressiveness of DGC cells. A Representative images and graphs of the transwell migration assay of SNU601 cells with or without fibroblasts (magnification, × 100). Data were analyzed using the Kruskal–Wallis test with Dunn’s test. B Representative images and graph of the soft agar assay of SNU601 cells with or without fibroblasts. The number of spheroids and percentage area were counted using the ImageJ software. Data were analyzed using ANOVA with Bonferroni correction. C Representative images and graphs of the soft agar assay of SNU601 cells with or without recombinant TINAGL1 treatment (rTINAGL1). Data analyzed using the Wilcoxon test. D Representative images and graphs of intraperitoneally injected SNU601 xenograft tumors (n = 6 per group; arrow, tumor nodules). Data were analyzed using the Kruskal–Wallis test with Dunn’s test. E, F Representative images of subcutaneously injected SNU601 xenograft tumors (E) and H&E staining of the entire tumor and cytokeratin staining of harvested inguinal lymph nodes (F). Scale bars, 1000 μm. G Pie chart shows the number of tumors with lymph node metastasis. Data were analyzed using Fisher’s exact test. *P < 0.05, **P < 0.01