Fig. 2

TINAGL1 interacts with integrins and activates the FAK signaling pathway. A Western blotting for integrin subunit levels in MKN45, KATO-III, SNU601, and SNU668 cells. B Representative images of immunocytochemistry staining for integrin β1 (green) in DGC cell lines (blue, nucleus; scale bars, 20 μm). C Immunoprecipitation assay was performed in SNU601 cells treated with recombinant TINAGL1 (rTINAGL1) or co-cultured with CAF47 (CAF). D Phospho Explorer antibody array for NF47- or CAF47-co-cultured SNU601 cells (orange, fold-change > 1; red, fold-change > 1.3). E, F Western blotting for FAK phosphorylation in fibroblasts co-cultured (E) or CM-treated (F) SNU601 cells. G Western blotting for FAK phosphorylation in fibronectin (FN) or TINAGL1-treated SNU601 cells