Fig. 1

TINAGL1 is a CAF-specific secretory molecule in DGC. A The heatmap represents the top-40 CAF-up-regulated genes, which were commonly increased in three NF-CAF pairs (fold-change > 2). B Gene set enrichment analysis for CAF-up-regulated genes. C Proteomic analysis for paired NF- and CAF-conditioned media. The red box indicates the enriched proteins in CAFs exhibiting over twofold-change. D The Venn diagram shows CAF-specific molecules up-regulated at the gene and protein levels simultaneously. E–H TINAGL1 gene and protein levels were validated in NF-CAF pairs by RT-PCR (E), western blotting (F), qRT-PCR (G), and ELISA (H)