Fig. 3

CDO1 predicts well prognosis of HCC patients and inhibits tumor progression. A CDO1 expression in HCC and non-tumor tissues and its correlation with HCC signatures in different datasets. Down, low expression in HCC tissues. B Correlation of CDO1 expression with overall survival and disease-free survival in 14 datasets. The X-tile method was used to find the best cutoff value. C Plasmid transfection up-regulates CDO1 expression in HCCLM3 and Hep3B cells. D CCK-8 assays for HCCLM3 and Hep3B cells transfected with OE-CDO1 or the control. E Cell apoptosis was measured by Hoechst-PI staining assays in HCCLM3 and Hep3B cells transfected with OE-CDO1 or the control. F Cell cycle analysis using PI staining in HCCLM3 and Hep3B cells transfected with OE-CDO1 or the control. G Transwell invasion assays for HCCLM3 and Hep3B cells transfected with OE-CDO1 or the control. Scale bars = 100 μm. H Wound-healing migration assays for HCCLM3 and Hep3B cells transfected with OE-CDO1 or the control. Scale bars = 200 μm. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. NA: not available