Fig. 2

ZIKV-LAV infection inhibits clonogenic reproduction of human GBM cells. Human GBM cells (DBTRG, LN18, and T98G) were inoculated with 1 pfu per cell of either ZIKV-LAV strains (DN-1 and DN-2) or parent HPF strain. Cells were trypsinized and re-seeded at 5,000 cells per well on day 5 post-infection. A Representative clonogenicity plates of infected cells. B Quantification of clonogenicity data, which are presented as mean ± SEM. Non-parametric Kruskal–Wallis test with Dunn’s post-hoc correction was used to compare groups. p-values are shown accordingly: *p < 0.05. **p < 0.005, ***p < 0.001