Fig. 7

The regulatory relationship between MEG3, hsa-miR-885-5p, and VASH1. A The relationship between MEG3 and hsa-miR-885-5p. MEG3 sponges hsa-miR-885-5p at certain sites. B The correlation between hsa-miR-885-5p and VASH1. Hsa-miR-885-5p targets VASH1 at certain sites. C MEG3 expression is negatively correlated with the expression of hsa-miR-885-5p. D Hsa-miR-885-5p expression is negatively correlated with the expression of VASH1. E MEG3 is mainly expressed in the cytoplasm. F, G The luciferase activity of the hsa-miR-885-5p mimic + MEG3-Wt and hsa-miR-885-5p mimic + MEG3-Mut groups was compared with that of miR-NC group in A2780 and SKOV3 cell lines. H, I The impacts of MEG3 expression on hsa-miR-885-5p in A2780 and SKOV3 cell lines. J, K qRT‒PCR validation of the effects of the hsa-miR-885-5p mimic and inhibitor on the expression of hsa-miR-885-5p. L, M Hsa-miR-885-5p hardly affected MEG3 expression. N, O The luciferase activity of the hsa-miR-885-5p mimic + VASH1-Wt and hsa-miR-885-5p mimic + MEG3-Mut groups was compared with that of the miR-NC group in A2780 and SKOV3 cell lines. P, Q The impacts of hsa-miR-885-5p mimic and inhibitor treatment on VASH1 expression were examined by qRT‒PCR and R, S western blotting in A2780 and SKOV3 cell lines. **P < 0.01; ***P < 0.001; ****P < 0.0001