Fig. 6

Characteristics of cancer cells in obese CRC. A UMAP plots of the epithelial cell, colored according to inferCNV inferred results. B Violin plots showing the expression of DEGs in malignant epithelial cells, colored by groups. C Violin plots showing the invasive characteristics score in malignant epithelial cells. Comparisons were performed by unpaired two-tailed Student’s t-test. Significance levels are expressed as *P < 0.05, **P < 0.01 and ***P < 0.001. D Heatmap showing the metabolic activity score of malignant epithelial cells by GSVA. The color darkness represents the scaled metabolic score. E Violin plots showing the expression of metabolism related DEGs in malignant epithelial cells, colored by groups. F Representative images of IHC staining detecting LDHA and SCD expression in obese CRC and non-obese samples. Scale bar, 20 μm. G The number of intercellular communications among different cell types in obese CRC and non-obese CRC samples. The line color represents cell types, and the line thickness represents interaction numbers. H Heatmap showing the overall CCL signaling by aggregating outgoing and incoming signaling together in the obese CRC and Non-obese CRC groups. I Dot plots showing the comparison of communication probabilities from malignant epithelial cells to six endothelial cell subsets among different groups