Fig. 2

Myeloid leukemia induces CAR down-regulation in different cell lines A, Several myeloid and B-lineage tumor cell lines used for in vitro culture are described in terms of their cell source, natural antigen expression and over-expression. B, Surface CD33 CAR expression and cytotoxicity of CAR T cells that co-cultured with Nalm6^CD33, HL60^CD33 and THP-1^CD33 at a low E: T ratio of 1:15. C, Surface CD33 CAR expression and cytotoxicity of CAR T cells that co-cultured with Nalm6^CD33, U937^CD33 and U937^CD33KO−OE. D, Surface CD123 CAR expression of CAR T cells that co-cultured with Nalm6^CD123, Raji^CD123, U937^CD123, MOLM-13^CD123 and THP-1^CD123 at a low E:T ratio of 1:15. Cytotoxicity of CD123 CAR T cells that co-cultured with Nalm6^CD123, Raji^CD123, U937^CD123, MOLM-13^CD123 and THP-1^CD123. E, Surface CLL-1 CAR expression and cytotoxicity of CAR T cells co-cultured with Nalm6^CLL−1, U937^CLL−1, HL60^CLL−1 and THP-1^CLL−1 at a low E: T ratio of 1:15. B–E, were representative data from at least three independent experiments. Assays were performed on day 10 after the initial T-cell culture. Unless otherwise noted, P values are determined by unpaired t-tests. The numbers on the graphs are P values