Fig. 2
From: Red blood cell-derived arginase release in hemolytic uremic syndrome
Mouse model of EHEC infection: clinical disease, survival and kidney injury. BALB/C mice were inoculated intragastrically with E. coli O157:H7 or PBS vehicle and when signs of disease developed or at end of experiment (days 6–9) blood samples and kidneys were collected. A Body weight change in mice inoculated with E. coli O157:H7 (n = 13) or vehicle controls (n = 8) presented as medians. B Survival plot of the same mice as in A. C Platelet counts in mice infected with E. coli O157:H7 (n = 13, median 486 × 109/L) and PBS vehicle controls (n = 8, median 662 × 109/L). D Neutrophil counts in mice infected with E. coli O157:H7 (n = 13; median 5.0 × 109/L) and PBS (n = 8; median 1.3 × 109/L). E Plasma urea in mice inoculated with E. coli O157:H7 (n = 10; three samples not analyzed, median 285 mg/dL) and PBS controls (n = 8, median 60.7 mg/dL). Bars denote medians. F A representative kidney section from a mouse infected with E. coli O157:H7. Arrowheads indicate tubular vacuolization. G Representative kidney section from a mouse infected with E. coli O157:H7. Arrowheads indicate tubular epithelial desquamation. H Representative kidney section from a mouse infected with E. coli O157:H7. Arrowheads indicate interstitial edema. I Representative kidney section from a control mouse showing normal mouse kidney tissue. Scale bar: 100 µm. Comparisons performed using Mann–Whitney U test (panels C-E). EHEC Enterohemorrhagic E. coli, PBS phosphate-buffered saline