Fig. 2

Characterization of HeLa derived-EVs. A Protocol of extracellular vesicles (EVs) production and isolation from HeLa cells. AB: apoptotic bodies, MV: microvesicles, Exo: exosomes. B Ponceau Red (tops) and western blot of tetraspanins (CD9 and CD81) and major volt protein (MVP) in large (lEVs) and small (sEVs) EVs isolated from HeLa conditioned culture media. C Nanoparticle tracking analysis (NTA) of isolated lEVs and sEVs quantifying their size distribution (top) and number of particles produced by cell (bottom). Statistical significance was determined by Wilcoxon-Mann Whitney test *p < 0.05. Data are obtained from 4 independent experiments. D Schematic representation of genomic and lncRNA sequences of LIPCAR. The black and red arrows correspond respectively to sequences amplified by LIPCAR-1 and LIPCAR-2 pairs of primers. E Quantification by qRT-PCR of LIPCAR levels in large (lEVs) and small (sEVs) extracellular vesicles isolated from HeLa conditioned culture media by using LIPCAR-1 and LIPCAR-2 primers (n = 6/group). GAPDH was used for normalization. Statistical significance was determined by Wilcoxon-Mann Whitney test and significant P values are indicated