Fig. 7
Transcription factor analysis of 4252 cells from 9 tumor cell clusters. A. Heatmap showing transcription factor activity enriched in different cell clusters. B. Regulatory network of transcription factors with targeted regulatory effects on genes by SPP1-(ITGAV +  + ITGB1) L-R pairs. C. AUCell quantification of POU5F1 transcription factor activity. D. Box plot illustrating significantly higher expression of POU5F1 in tumor cells than in epithelial cells (Mann–Whitney U test, ***p < 0.001). E. Multiplex immunofluorescence staining in ccRCC samples and in normal kidney tissues for verification of SPP1, POU5F1 and CD44 gene expression. Renal tubular epithelial cells arranged in a ring-like pattern in normal kidney tissue