Fig. 5
DX2 restores motor symptoms in MPTP-induced PD model A Scheme of scAAV2-DX2 transduction in MPTP-induced mouse model. scAAV2-DX2 was infused in the substantial nigra region with the stereotaxic machine and then subcutaneous injection with MPTP was performed once per day. Before measuring the behaviour test, the training of each test was continued. B scAAV2-DX2-treated mice showed slightly longer latency to fall in the rotarod test when compared with vehicle (scAAV2-GFP, GFP), indicating that scAAV2-DX2 attenuated damage towards dopaminergic neurons. C DX2-treated mice showed improved locomotor activity based on the SHIRPA test. D DX2-treated mice showed a relatively lower level of limb deficit. E Immunofluorescence image of TH-positive cells in the mouse substantia nigra. The white scale bar represents 500 µm F, G DX2 F and Bax G mRNA expression of the indicated mice brain. H–I Quantification of immunoblot assay for cleaved caspase-8 and cleaved caspase-9. animals: naive, n = 6; GFP, n = 9; DX2, n = 12. scAAV2: scAAV2-GFP, 4 × 109 vg; scAAV2-DX2, 4 × 109 vg. ns, non-significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; t-test