Fig. 3

Development of scAAV-DX2 with the microRNA142-3p target sequence A Screening of hematopoietic cell expressed microRNA. Among the miRNA, miR142-3p was more highly expressed than the others B The relative value of Luciferase assay result with hematopoietic cell expressed miRNA and its target plasmid. P Plasmid. C Scheme of DX2 vector with variously repeated miR142-3p target sequence and mutation form. The symbol of * is a single mutation sequence. D, E DX2 expression is inhibited by the miR142-3p target sequence in hematopoietic cells. F Scheme of scAAV-DX2 with miR142-3p target sequence. ns: no-significant; *: p < 0.05; **:p < 0.01; ***:p < 0.001. t-test. TS: miR142-3p target sequence; mut: mutation