Fig. 2
Efficacy test of scAAV2-GFP and scAAV2-DX2 in vitro and in vivo A, B Transduction efficacy test of AAV2-GFP in Hela, Hek293 and SK-N-SH. A 1000MOI concentration of ssAAV2-GFP and scAAV2-GFP B 10000MOI cencentration of ssAAV2-GFP and scAAV2-GFP C GFP expression was observed with fluorescence microscopy in SH-SY5Y. D The percentage of GFP-positive cells was measured following dose-dependent infection for 48 h with ssAAV2-GFP and scAAV-GFP in the SH-SY5Y cell line. E scAAV2-GFP expression in the right striatum, scale bar: 500 μm and 100 μm. F scAAV2-GFP expression in the spinal cord of intrathecally injected mice. The lumbar region specified as L1 to L5 was injected and used for confocal imaging. G DX2 expression was checked on the day after injection at the isolated substantia nigra using real-time qPCR. After scAAV2-DX2 treatment in H2O2-induced apoptosis situations. H The difference in AIMP2 and DX2 expression levels in mouse organ