Fig. 1

Protective effect of DX2 against ROS-induced cell death in SH-SY5Y cells. A A graph showing a viable cell counting in SH-SY5Y cells overexpressing Flag-EV, Flag-AIMP2, and Flag-DX2, after screening with cell death-inducing chemicals. All chemicals were treated for 24 h after vector overexpression, at the following concentrations: H₂O₂ (100 μM), 6-OHDA (100 μM), TNF-α (10 μM), cycloheximide (CHX, 10 μM), actinomycin D (Act D, 10 nM), cisplatin (Cis, 10 μM), paclitaxel (Pac, 10 μM), and 5-fluorouracil (5-FU, 10 μM). B A graph comparing cell counts between AAV-GFP and AAV-DX2 infections. All cell countings were repeated three times. C–E Quantitative PCR graphs analyzing the expression patterns of mRNAs involved in cell survival by AAV-DX2 F–I Immunoblot assays confirming the Caspase-cascade pathway in cell survival induced by AAV-DX2. J Quantification of dot blot assay data for AAV-GFP and AAV-DX2