Fig. 3

ChemR23 deficiency promoted neuronal pyroptosis in MCAO mice. A, B Western blot and quantitative analysis of GSDMD-N at Day 1 after MCAO (n = 3/per group). C, D Double immunostaining of GSDMD with NeuN in ischemic penumbra region and quantitative analysis at Day 1 after MCAO (n = 3/per group). Scale bar = 20 μm. E, F ELISA analysis for IL-1β and IL-18 levels in ipsilateral brain tissues (n = 4/per group). G Representative transmission electron microscopy pictures of neurons in ischemic penumbra. Pores on neuronal membrane are pointed out with red arrows (n = 3/per group). Scale bar = 2 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001