Fig. 7

PLOD3 accelerated the progression of CRC via TNFα/NF-κB signaling pathway. A Heatmap representation of genes differentially expressed in PLOD3-knockdown HT29 cells identified by RNA-seq. B GO analysis of the genes that were uniquely downregulated. C qRT-PCR analysis of TNF-a, IL-6 and IL-1b mRNA levels normalized to GAPDH in the CRC cell lines stably transduced with PLOD3-targeting shRNA or control. D Immunofluorescence staining of NF-κB (Red) and DAPI (Blue) in MC38 and SW480 cells analyzed by Single Photon Laser Scanning Confocal Microscopy. Magnification, × 160. E Luciferase reporter assay indicated that the knockdown of PLOD3 suppressed the p-NF-κB luciferase activity in HT29 cells. (F) Western blot to measure the protein expression of NF-κB pathway after PLOD3 knockdown. Data are shown as the mean ± SEM (three independent experiments). *P < 0.05; **P < 0.01; ***P < 0.001