Fig. 4

Facilitating PKM2 tetramerization promoted mitochondrial network formation. A Rat primary cardiomyocytes were treated with AVP and TEPP-46 as indicated and stained by MitoTracker-Red to visualize mitochondrial morphology. Scale bar = 20 µm. B-E The means of area, perimeter, aspect ratio and form factor of mitochondria were quantified. Results are expressed as mean ± SEM, n = 25 cells per group. F–I Lysates from primary cardiomyocytes were analyzed by western blotting with indicated antibodies. Relative protein level was quantified and expressed as mean ± SEM, n = 3