Fig. 2

Quantitative proteomics reveals that PKM2 is upregulated during right ventricular failure and negatively correlated with the cardiac function. A The volcano plot of proteins quantified by mass spectrometry from right ventricular tissue of control and RVF rats. B Relative abundance of proteins known to be involved in heart failure from mass spectrometry. The dash lines represent the upregulation and downregulation thresholds. C–F Heatmaps of differentially expressed proteins related to fatty acid beta-oxidation, TCA cycle, OXPHOS and glycolysis from mass spectrometry. G–H Lysates from right ventricle of control and RVF rats were analyzed by western blotting with indicated antibodies. Relative protein level was quantified and expressed as mean ± SEM, n = 4 rats in control group, n = 10 in RVF group. I The relative protein level of PKM2 was negatively correlated with the right ventricular function