Fig. 2

Ozone activated the AMPK/SOCS3 signal pathway and inhibited MMP9 expression to relieve gout pain. A After 6 h of indicated treatments, the MMP9 of mice 's plasma was tested using western blot. Albumin was used as a loading control. B MMP9 were measured with zymography assays. Blood cells (C, D) and ankle joints (E)with the indicated treatments were subjected to western blot assay of pAMPK, AMPK, SOCS3, MMP9 and β-actin. F–H Up/down-regulation of IL-1β, TNF-α and IL-6 mRNA were compared with the control group and MSU-treated group (n = 4). I Effect of ozone on MSU-induced mechanical withdrawal threshold combined with CC-treatment in GA mice. CC (i.p, 5 mg/kg) were primed 12 h before MSU injection. J Joint swelling gain at different time points. K, L Western blot analysis and the expression of MMP9 and SOCS3 in ankle joint. Representative western blot bands and a data summary are shown. ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs. control group; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 vs. MSU-treated group; ^&p < 0.05, ^&&p < 0.01 and ^&&&p < 0.001 vs. MSU + ozone group