Fig. 1

Ozone suppressed MSU-induced gout pain in mice. Mice (n = 8) were treated with various doses of ozone (i.p) 10 min after the injection of MSU crystals (0.5 mg/10 μl). A Mechanical allodynia was performed to evaluate the effect of ozone. B Time course of changes in MSU-induced ankle swelling. C Representative photographs and hematoxylin- and eosin-stained sections of the ankle joints obtained 6 h after MSU injection. D Representative footprint images were recorded during the treatment of Ozone. The colored bands represent standing time of each foot and the right panels are footprints of the right hind paw reflected by green LED light. E, G, I The paw mean intensity was analyzed by CatWalk software. RH, right hind. F, H, J The duty cycle was analyzed by CatWalk software. RH, right hind. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. sham group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the MSU-treated group