Fig. 5
From: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells
RUNX1 knockdown affects EMT in ovarian cancer cells via the EGFR-AKT-STAT3 axis. Western blot detected the signaling molecules change pattern in the RUNX1 KD cells that involved in the EMT related signaling pathway (A and B). Western blot detected the signaling molecules change pattern after Ro5-3335 (a RUNX1 inhibitor) treatment that involved in the EMT related signaling pathway (C and D). Western blot detected of EMT-related molecules in RUNX1 knockdown cell lines (E and F). Western blot detected of EMT-related molecules after Ro5-3335 treatment (G and H). The expression of EMT-related molecules was analyzed by immunofluorescence in RUNX1 knockdown cell lines (I and J). *p < 0.05, **p < 0.01 and ***p < 0.001 as compared with control cells expressing a scramble shRNA control, paired t test