Fig. 4
From: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells
The RUNX1 knockdown impacted apoptosis in the ovarian cancer cell via the FOXO1-Bcl2 axis. GSEA identified the enrichment plot of apoptosis-related genes in RUNX1 KD compared to control cells (A). Heat maps significantly compare the gene expression involved in apoptosis regulation in RUNX1 KD and control groups (B). Annexin V-PI staining analysis by flow cytometry showed that the knockdown of RUNX1 enhanced the apoptosis level of ovarian cancer cells (C and D). Western blot detected apoptosis-related molecules in RUNX1 knockdown cell lines (E and F). Western blot detected of apoptosis-related molecules after Ro5-3335 treatment (G and H). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to control cells expressing a scramble shRNA control, paired t-test