Fig. 2

The RUNX1 mediated the abnormal proliferation, invasion, and migration in the ovarian cancer cells. Western blot confirmed RUNX1 stable knockdown in SKOV3 cells constructed by plasmids containing RUNX1-targeting shRNA (A). CCK8 assay showed that knockdown of the expression levels of RUNX1 reduced the proliferation ability of SKOV3 (B). Western blot confirmed RUNX1 stable knockdown in OVCAR3 cells constructed by plasmids containing RUNX1-targeting shRNA (C). CCK8 assay showed that knockdown of the expression levels of RUNX1 reduced the proliferation ability of OVCAR3 (D). Differences in the migration and invasion ability of cells after the knockdown of RUNX1 expression levels and the migration and invasion ability of the cells were decreased when RUNX1 was knocked down (E and F, 200 ×). Colony formation assay showed that knockdown of the expression levels of RUNX1 reduced the ability of colony formation of SKOV3 and OVCAR3 (G and H). A representative experimental result was generated from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to control cells expressing a scramble shRNA control, paired t-test