Fig. 8

CircKIF18B_003 and ACACA Regulate Lipid Metabolism in Prostate Cancer Cells. A–D The formation of fatty acids (FA) and triglycerides (TG) in prostate cancer cells is regulated by circKIF18B_003. In DU145 cells, knockdown of circKIF18B_003 decreases the levels of these lipids, while in PC-3 cells, overexpression of circKIF18B_003 increases their levels. E Overexpression of circKIF18B_003 in PC-3 cells greatly enhances the incorporation of glucose into FA, as evidenced by liquid chromatography-mass spectrometry (LC–MS) analysis. F–J The glucose-incorporating effect of circKIF18B_003 overexpression is reversed by either knockdown of ACACA or treatment with ND630, suggesting a regulatory role of ACACA in lipid synthesis. K Knockdown or inhibition of ACACA by siACACA in DU145 cells significantly decreases the incorporation of glucose into de novo FA synthesis. These results collectively suggest a pivotal role of circKIF18B_003 and ACACA in enhancing glucose utilization for FA synthesis in prostate cancer cells, thereby contributing to the malignant phenotype of these cells. *P < 0.05, **P < 0.01, ***P < 0.001