Fig. 2

DCZ5417 inhibits multiple myeloma. a Indicated MM cell lines were treated with vehicle or DCZ5417 at the indicated concentrations for 72 h. Then the cell viability was determined by CCK-8 assay. Cell viability data are presented as the means of 3 independent experiments in a Heatmap. b Soft agar colony formation of H929 and OCI-MY5 cells were tested after DCZ5417 or DMSO treatment. Quantification of the colony numbers in the left. *P < 0.05. c H929 and OCI-MY5 cells were exposed to 0 and 10 µmol/L DCZ5417 for 48 h, Edu assay was carried out. Statistics of Edu-positive cells in the left. d Representative fluorescent images of typical apoptotic cells evaluated by TUNEL staining (red) after 10 µmol/L DCZ5417 treatment for 24 h. DAPI was used as a nuclear stain (blue). e Flow cytometry evaluation of Annexin-V-positive apoptotic cells in DCZ5417-treated H929 cells. Representative results of triplicate experiments were shown. f Immunoblotting was used to analyze the expression levels of Caspase-3, Caspase-8, Bax, and Bcl-xL. g Cell-cycle analysis of DCZ5417 (0, 5, and 10 µmol/L, 24 h)-treated H929 and OCI-MY5 cells. h Effects of DCZ5417 treatment on CDK4, CDK6 and Cyclin D1 expression in cells. Data are presented as the means ± SD of 3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus the control group