Fig. 5
Post-thaw characterization of aged products cryopreserved at the end of their shelf life (72 h post-apheresis). The effect of a freeze–thaw procedure after storage at -180 °C for at least 1 month was analyzed on A aged CD45RA-depleted DLI product and B aged TCRαβ/CD19-depleted HSPC graft regarding the parameters CD45 viability, viable CD45 recovery, CD3 recovery or CD45 viability, viable CD45 recovery, CD34 viability and viable CD34 recovery, respectively. Individual runs depicted as dots as well as mean ± SEM are shown; n = 4 of each independent cryopreserved products of aged TCB1 and aged TCB2 from 4 freshly processed mobilized apheresis products per depletion process. Recovery values were calculated compared to the corresponding pre-cryopreserved fresh product