Table 1 Sequences of primers, probes and shRNAs
From: A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification
| Â | Gene symbol | Sequences |
|---|---|---|
qPCR primers | Human TRIM59 | F: 5′-CCTGCCCTGAACATTAC-3′ |
|  | R: 5′-GCTTCCTTATCGCCTTG-3′ | |
Rat TRIM59 | F: 5′-TCAGGCATCTGGTAACTT-3′ | |
|  | R: 5′-ACATCTGGGTGGTCTTC-3′ | |
Human YAP1 | F: 5′-TGACCCTCGTTTTGCCATGA-3′ | |
|  | R: 5′-GTTGCTGCTGGTTGGAGTTG-3′ | |
Human TEAD4 | F: 5′- GGCACCATTACCTCCAACG-3′ | |
|  | R: 5′-TGTAGCGGGCAATCAGC-3′ | |
ChIP primers | TRIM59 promoter (− 1703 ~ − 1692) | F: 5′-TTAAGGAGCTGAATTGGT-3′ |
|  | R: 5′-GAGAATCGCTTGAACCC-3′ | |
TRIM59 promoter (− 1186 ~ − 1174) | F: 5′-GAGGACCAAACCATACCA-3′ | |
|  | R: 5′-TAGCCACCCAGGAGACAC-3′ | |
Oligonucleotide pull-down probes | WT probe | 5′-GGAGTAACAAAATACTTTTTCTTGGAATGTAGTAATCTGTAACCTACCAAATA-3′ |
MUT probe | 5′-GGAGTAACAAAATACTTTTTGTAGCATTCTTGTAATCTGTAACCTACCAAATA-3′ | |
 shRNAs | Human YAP1 shRNA targeted sequence | 5′-ATTTAAGAAGTATCTCTGACC-3′ |
Human TEAD4 shRNA targeted sequence | 5′-TGTTGGTGTTGAGGTCTGCCC-3′ | |
Human TRIM59 shRNA-1 targeted sequence | 5′-TCTAATGTATCATTAAGCTCC-3′ | |
Human TRIM59 shRNA-2 targeted sequence | 5′-ATTAATGTAACTACAACAATG-3′ | |
Rat TRIM59 shRNA targeted sequence | 5′-GTTAACTGTTTAAACAACTTC-3′ |