Fig. 4

TRIM59 silencing remitted PH. A SuHx rat model of PH was established following infection with AAV2 carrying TRIM59 shRNA, then the mRNA level of TRIM59 was determined by qPCR. B Western blot was conducted to detect the TRIM59 protein level after TRIM59 silencing in PH rats. C After TRIM59 silencing, the right ventricular systolic pressure was measured. D HE staining was conducted to evaluate the histopathological changes of heart. Magnification: × 200. E HE staining was performed to assess the histopathological changes of lung. Magnification: × 200. F Immunohistochemical staining was performed to detect the level of α-SMA. Magnification: × 200. G Imunofluorescence staining with TRIM59 and α-SMA was performed. Magnification: 100 ×, 400 ×. H The level of α-SMA and Ki-67 was determined by imunofluorescence staining. Magnification: 400 ×. * p < 0.05