Fig. 2

TRIM59 was highly expressed in the lung tissues of rat model and cell model of PH. A A rat model of PH was established via a single subcutaneous injection of Sugen-5416 (20 mg/kg) and fed in a hypoxic condition. B The right ventricular systolic pressure was measured via right heart catheterization. C Immunohistochemistry staining was performed to detect the expression and distribution of TRIM59. Amplification: 100 × and 400 ×. D The expression of TRIM59 in the lung tissues of PH rats was determined by qPCR. E Western blot was performed to detect the TRIM59 expression in the lung tissues of PH rats. F Double immunofluorescence staining was performed to detect the TRIM59 expression and distribution in lung tissues. Amplification: 100 × . G Human PASMCs were cultured in hypoxic condition for 24 h. H The level of TRIM59 was detected by western blot. I qPCR was conducted to determine the expression of TRIM59 in PASMCs under a hypoxic condition. *p < 0.05