Fig. 6
Animal Experiment Verification A. Schematic diagram showing the workflow of animal experiments. A total of 5 × 105 (50 µl) mouse gastric cancer cells (MFCs) were injected into the subcapsule of the spleen of 8 mice (615 Mouse). Seven days after tumor development, four mice in the experimental group were intraperitoneally injected with a GDF15 inhibitor, and four mice in the control group were intraperitoneally injected with an equal volume of PBS. Thereafter, treatments were performed every 3 days for a total of 6 injections. After the last treatment, the mice were euthanized the next day, and liver metastases were collected. B. In vivo fluorescence imaging shows the formation of liver metastases from gastric cancer in mice. C. Multiplex immunofluorescence image showing the colocalization of macrophage markers (Cd68), SPP1 + TAM markers (Spp1) and Gdf15 protein. D. GDF15 inhibitors reduced the number of liver metastases (each group contained 4 mice). E. Immunohistochemical image of HE staining showing that GDF15 inhibitors can reduce the number of liver metastases. F. Immunofluorescence image showing that GDF15 inhibitors increase tumor-infiltrating CD8 + T cells (each group contained 4 mice). G. Flow cytometry showing that GDF15 inhibitors increase tumor-infiltrating CD8 + T cells (each group contained 4 mice). The number of liver metastases and flow analysis were performed by Student’s t test, and P < 0.05 was considered statistically significant