Fig. 4

Interaction between macrophages and CD8 + T cells A. Heatmap showing the strength of intercellular communication in different disease states. The redder the color is, the stronger the communication between cells. The strength of the crosstalk between CD8T-C4 (exhausted CD8 + T state) and MACRO-SPP1 (SPP1 + TAMs) was significantly enhanced in the metastatic state. B. Incoming and outgoing communication strength of each cell subset in different disease states. The outgoing strength of MACRO-SPP1 (SPP1 + TAMs) was higher than the incoming strength. The incoming strength of CD8T-C4 (exhausted CD8 + T state) was higher than the outgoing strength. MACRO-SPP1 (SPP1 + TAMs) are more likely to be outcoming cells. CD8T-C4 cells (exhausted CD8 + T cells) are more likely to be incoming cells. C. Heatmap showing detailed intercellular signaling pathways between macrophages and CD8 + T cells in different states. D. Histograms showing signaling pathway changes from output MACRO-SPP1 (SPP1 + TAMs) to input CD8T-C4 (exhausted CD8 + T state) in different disease states. The VCAM, GDF, MIF, TNF, CD137 and ITGB2 pathways significantly appear in metastasis. E. Hierarchical diagram visually showing the interaction of the above signaling pathways in metastasis. The GDF signaling pathway was only specifically sent by MACRO-SPP1 (SPP1 + TAMs)