Fig. 3

Subclustering and annotation of macrophages. A. UMAP diagram showing 5 major macrophage subsets. Based on their type-specific markers, we named them MACRO-C1QC, MACRO-SPP1, MACRO-FCN1, MACRO-GBP1, and MACRO-INHBA. B. UMAP diagram showing classic characteristic marker gene expression of 5 major macrophage subsets. C. Bubble heatmap showing the expression of major type-specific markers in different macrophage subsets. D. Line graph showing disease state preference for different macrophage subsets. MACRO-SPP1, MACRO-INHBA, and MACRO-GBP1 subgroups were not found in normal gastric tissue. These 3 cell types displayed tumor-associated macrophages. MACRO-SPP1 cells were defined as SPP1 + TAMs. E. Boxplot showing the functional characteristic scores of different tumor-associated macrophage subsets. F. Heatmap showing M1/M2 signature scores and expression of M1/M2 signature genes for different macrophage subsets. G. Scale diagram showing different macrophage subset cellular compositions in different disease states. Wilcoxon test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.